Optimal Temperature and pH Pattern for the Cultivation of Temperature Inducible Gene Engineered Escherichia coli Utilizing Genetic Algorithm.
نویسندگان
چکیده
منابع مشابه
Evolutionary changes in heat-inducible gene expression in lines of Escherichia coli adapted to high temperature.
The involvement of heat-inducible genes, including the heat-shock genes, in the acute response to temperature stress is well established. However, their importance in genetic adaptation to long-term temperature stress is less clear. Here we use high-density arrays to examine changes in expression for 35 heat-inducible genes in three independent lines of Escherichia coli that evolved at high tem...
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Background The Hormone Group of IPEN develops researches on recombinant pituitary hormones produced in Escherichia coli at laboratory scale, including human growth hormone (hGH) and prolactin (hPRL). The best results were obtained using the lPL promoter and the signal peptide DsbA, and so the protein of interest is secreted into the bacterial periplasmic space, in its authentic form without an ...
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Objectives/Goals The purpose of this project was to study the growth of the bacteria Escherichia coli DH5a under a variety of different pH and temperature, in order to discover and approximate the optimal value at which the cell was able to increase in size and divide the quickest. Methods/Materials Material used for this experiment included Eschericha coli DH5a, flasks, cuvettes, test tubes, a...
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2.Bailey, J.E., N.A. Da Silva, S.W. Peretti, J.H. Seo and F. Srienc. 1986. Studies of hostplasmid interactions in recombinant microorganisms. Ann. NY Acad. Sci. 469:194-211. 3.Lerner, C.G. and C.D. Carter. 1995. PCR colony screening using the scintillation proximity assay to detect inserts in cloning vectors. BioTechniques 19:914-917. 4.Liu, Z. and N.C. Mishra. 1995. Single-tube method for plas...
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Successful germination is the main factor in proper establishment and seedling yield. Information on seed germination, in the face of temperature changes at the time of planting, is a way to identify more suitable cultivars for cultivation at the planting site. For this purpose, a factorial experiment was performed based on completely randomized design with 3 replications. This experiment was c...
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ژورنال
عنوان ژورنال: KAGAKU KOGAKU RONBUNSHU
سال: 1996
ISSN: 0386-216X,1349-9203
DOI: 10.1252/kakoronbunshu.22.1391